Innovative Methodology Rapid screening of glomerular slit diaphragm integrity in larval zebrafish
نویسندگان
چکیده
Dirk M. Hentschel, Michael Mengel, Lisa Boehme, Fabian Liebsch, Caroline Albertin, Joseph V. Bonventre, Hermann Haller, and Mario Schiffer Renal Division, Department of Medicine, Brigham and Women’s Hospital, Boston, Massachusetts; Department of Pathology and Division of Nephrology, Department of Medicine, Hannover Medical School, Hannover, Germany; and Mount Desert Island Biological Laboratories, Salisbury Cove, Maine
منابع مشابه
Rapid screening of glomerular slit diaphragm integrity in larval zebrafish.
Gene array-type experiments have identified large numbers of genes thought to be important for the integrity of the glomerular slit diaphragm. Confirmation of individual proteins has been limited by the expenses and time involved in generating transgenic or knockout mice for each candidate. We present a functional screening assay based on the clearance of a 70-kDa fluorescent dextran in another...
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Morphine has been reported to accelerate the progression of chronic kidney disease. However, whether morphine affects slit diaphragm (SD), the major constituent of glomerular filtration barrier, is still unclear. In the present study, we examined the effect of morphine on glomerular filtration barrier in general and podocyte integrity in particular. Mice were administered either normal saline o...
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تاریخ انتشار 2007